Answer
I don't know what exactly constitutes statistically significant, but I'll take a stab at it. For whatever game you play, you have a certain win rate and standard deviation. After you play n number of hands, your expected win is EV/hand * n. Your standard deviation is SD/hand * sqrt(n).
If you are a basic strategy player, your EV/hand is about -.004 units(.4% house advantage), and SD/hand is 1.15 units. After you have played 10,000 hands at say, $10/hand, your EV is $-400, and SD is $1150, so your expected win/loss = $-400 +- $1150*SD. Now, after you have played 1,000,000 hands, your EV is $-40,000, and SD is $11,500, so your expected win/loss = $-40,000 +- $11,500*SD. You can see that as the number of hands go up, the SD/EV goes down. In the first case, the SD is 287.5% of the mean. In the second case, it is only 28.75%.
As a counter, your EV/hand may be something like .015 units, and SD/hand something like 2.80 units. When counting, many people like to refer to N0, which is a measure of how many hands it takes to overcome a negative fluctuation of one standard deviation. In other words, it is the number of hands that must be played so that EV = SD. To determine N0:
EV * N0 = SD * sqrt(N0)
sqrt(N0) = SD/EV
N0 = (SD/EV)^2
So in the example case, N0 = (2.80/.015)^2 = 34,850 hands. This says that after 34,850 hands, you will have earned money, unless you have suffered from greater than -1 SD's (16% chance). You may also determine how many hands it takes to overcome 2 and 3 negative standard deviations. These values are 4*N0 (139,400) and 9*N0 (313,650). This says that after having played 139,400 hands, you will have made a profit unless you have suffered from more than 2 negative SD's (about a 2.5% chance), and that after having played 313,650 hands, you will be on the plus side unless you have suffered more than 3SD's on the sad side (.15% chance).
To put these numbers into perspective, if you played a game with this EV and SD, 8 hrs a day and 5 days a week (100 hands/hr), for 35 weeks (about 139,400 hands), you would have a 1/40 chance of winning nothing or losing money. Then again, you would have the same chance of winning 4182 units (104K with a $25 unit). You can see that even though playing this number of hands gives you a sound chance of making money, you could win $5 or you could win $100K. This isn't something that I would want to have to rely upon to make serious money (aka professionally).
Hopefully this answered something...I'm sure there are others who could supply better answers. Good luck.
--Homer J.